Characterization of tospovirus isolates from Portugal
1999
Cortes, I. | Kormelink, R. | Peters, P. | Pereira, A.M.N.
Plants with typical symptoms of tomato spotted wilt virus were collected from Algarve and Ribatejo e Oeste regions, in 1997. The plant material was analised by DAS-ELISA against polyclonal anti N-sera to several tospoviruses species, however they reacted positivly just with TSWV antisera. Two isolates collected in Algarve from pepper and tomato, and one from Ribatejo e Oeste, from pepper, were sellected for a detailled study. These three isolates were characterized at molecular and biological level. The N gene of the SRNA was sequenced and the host range was determined. The molecular studies confirmed the results obtained with serology. The comparison of the aminoacids of the N gene of these portuguese isolates and TSWV revealed a high homology level between them. With these results, the portuguese isolates studied up to now are included on the TSWV species (tomato spotted wilt virus). The host range results however showed interesting features. These isolates from Portugal were more virulent, in comparison with TSWV, producing a quick and strong necrosis on the plants, as well as their quicker dead. Also, these isolates were able to induce systemic infection in plants that are referred as just developing local symptoms after inoculation with any of the tospoviruses
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