Structural changes of plasma membrane after incubation of boar spermatozoa for 24 hours at room temperature as judged by electron microscopy and lectin binding pattern
1994
Tamuli, M.K. | Watson, P.F. | Plummer, J.M. (Royal Veterinary College, London (UK). Dept. of Veterinary Basic Sciences)
Boar spermatozoa were investigated for their changes in the membrane structure after incubation for 24 h at room temperature (18-20 deg C) following the evidence that they had a tremendous response (80-90 percent) for the development of resistance to cold shock. Electron microscopy studies indicated that the spermatozoa seems to undergo dehydration. This was evidenced with the condensation of acrosomal content seen with increase in density, a definite knob formation of the apical ridge from its turgid appearance before incubation and wrinkling of the plasma membrane irrespective of the region. Staining of carbohydrates of cell surface of Percoll washed spermatozoa by alcian blue suggested that the glycocalyx appeared to be more stable and there was slight increase in the concentration of carbohydrates evidenced with more uniform alcian blue pigments distribution on the membrane surface of spermatozoa. Fluorescein conjugated lectin binding of 24 h incubated spermatozoa resulted that concentration of WGA was slightly increased as seen from increase in fluorescence and the others, Con A, RCA-I, SBA and Lotus A were decreased. These changes suggested that plasma membrane became more stabilized after 24 h incubation at room temperature.
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