Mutation of Monascus sp. KB11304 for albino mutant and its comparative studies to the wild type, red and yellow mutants
1994
Nisa Budda
Monascus sp. KB20M1, an albino mutant, was derived from the wild type, KB11304, by UV-irradiation for 20 min. The studies on morphology, physiology, capability on producing pigments, enzymes, ethanol, antibacterial agents as well as pigment characteristics were compared with those of strains KB10M16 (red mutant), KB20M10.2 (yellow mutant) and the wild type. An albino mutant, KB20M1 produced massive sporulating aerial white thin mycelium with lava-type forming colony on PDA agar similar to the wild type. Strains KB10M16 and KB20M10.2 produced dense downy mycelium with lava-type colony forming at young stage, but grew poorly on PDA agar and rarely formed spore. The wild type and strain KB20M1 grew very well in liquid medium such as SS, MYS and defined media, whereas the mutants, KB10M16 and KB20M10.2 grew well in SS and MYS media, but very poorly in defined medium. In solid-state cultivation using rice as substrate, the optimum initial moisture content for red and yellow pigments production was 38.0 percent. An albino mutant, KB20M1 could produce glucoamylase, alpha-amylase, ethanol and antibacterial agent better than the other strains, but produced acid-protease in comparable to strain KB20M10.2. Crude red pigments from wild type and strain KB10M16 were stable at pH range 5.0-10.0 and very stable when solubilized in ethanol, distilled water and glycerol. Crude yellow pigment from strain KB20M10.2 was stable at pH range 3.0-12.0 and stable when solubilized in distilled water, ethanol and 10 percent sodium chloride. Both red and yellow pigments were stable at temperature range as boiling, pasteurization and sterilization, but unstable to UV irradiation. The crude pigments were highly stable at 4 deg C or in the dark.
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