Congenital pestivirus infection in sheep, ewes, heifers and calves with Bovine pestivirus strains and detection of antigenic differences between them
1999
Dimitrova, E. | Martinov, S. (Central Research Veterinary Medical Institute, Sofia (Bulgaria))
Intranasally and intramuscularly inoculated five groups (A-E) of seronegative sheep, ewes, goats, cattle, heifers and caws at days 49th and 126th of gestation with either of four antigenically different BVDV reference strains (OREGON C 24, NADL, OSLOSS, ALFORT-187) and one BVDV Bulgarian strain (KABLESHKOVO-76). All ruminants of groups were seronegative in the ELISA and in the virusneutralization test ( beta-procedure). At 2 and 4 weeks after infection the virus was isolated from almost all foetal organs in the all groups inoculated with different pestivirus strains. In experiments the sheep, ewes, goats and saws were inoculated with antigenically heterologous stains at 49th day of pregnancy. Rectal temperature of sheep, goats, pigs and calves were recorded daily during 14 days, starting 2 days before inoculation and clinical observation of all ewes, saws and heifers were performed daily during the experiments.
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