Comparison of 3H-thymide incorporation and CellTiter 96TM aqueous colorimetric assays in cell proliferation of bovine mononuclear cells
1998
Zolnai,A. | T¢th,E.B. | Wilson,R.A. | Frenyo,V.L.
A rapid colorimetric non-radioactive assay for bovine mitogen-induced lymphocyte proliferation in cell culture was tested using a novel tetrazolium compound (MTS) and an electron coupling reagent (PMS) provided in the CellTiter 96TM kit (Promega).The results were compared with those of 3H-thymidine incorporation assay using samples from the same lymphocyte population.The concentrations used in the cell suspension of primary cultured lymphocytes gave a significant signal/background ratio when cells were prepared from peripheral blood, spleen or mesenteric lymph nodes.The same concentrations of thymocytes produced a weak signal even for the two methods.The non-radioactive method performed well in assay in bovine mononuclear cells derived from prolactin-treated calves, showing a 50% higher response to mitogenic stimulation than that in untreated calves.
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