Peripheral circulating insulin-like growth factor-I and -II in cattle
2001
Nikolic, Judit, Anna | Nedic, Olgica | Samanc, H. | Aleksic, S. | Miscevic, B. | Kulcsar, Margit
Interrelationships between circulating concentrations of the insulin-like growth factors (IGF-I and IGF-II) were investigated in 235 blood samples taken from 145 healthy beef or dairy calves, bulls and cows of different breeds and ages. Each IGF radioimmunoassay was validated by determining the effects of IGFBPs, ligand and contraligand, as well as serial dilution and comparison with results obtained after molecular sieve chromatography in acid. The tendency for a reciprocal relationship between serum concentrations of these growth factors was most obvious in the periparturient cows.
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