Efficient plant regeneration from protoplasts of sweet potato, Ipomoea batatas (L.) Lam
1998
Wang, J.S. (Kagoshima Univ. (Japan). Faculty of Agriculture) | Sato, M. | Taura, S. | Kokubu, T.
Protoplasts isolated from petioles of sweet potato cv. Genki and White Star and from embryogenic calli of cv. Bitambi were cultured in the modified liquid MS medium containing 0.05 mgl(-1) 2,4-D and 0.5 mgl(-1) kinetin. The first cell division occurred within 3 to 4 days. After 8 to 9 weeks of incubation, protoplast-derived calli up to 1-2 mm in diameter were plated on the solid MS medium supplemented with 2,4-D (0.05-0.2 mgl(-1)) and kinetin (0-0.5 mgl(-1)) for callus proliferation. Furthermore, the calli were transferred onto either the hormone-free medium or the medium supplemented with BAP (2-3 mgl(-1)), followed by being transferred onto the hormone-free medium for plant regeneration. The result showed that the callus proliferation medium supplemented with 0.05 mgl(-1) 2,4-D and 0.5 mgl(-1) kinetin was most efficient for plant regeneration in all the cultivars used, and the effect of BAP was varied with genotypes. BAP played an important role in promoting the plant regeneration in Genki, but inhibited the plant regeneration in White Star and Bitambi
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