Efficient transformation of Marchantia polymorpha [Bryophyta] that is haploid and has very small genome DNA
1997
Nasu, M. (Osaka Univ., Suita (Japan). Faculty of Pharmaceutical Sciences) | Tani, K. | Hattori, C. | Honda, M. | Shimaoka, T. | Yamaguchi, N. | Katoh, K.
The genomic DNA content of a cultured cell of Marchantia polymorpha HYA-2F was examined using a flow cytometer. It was estimated to be 0.32 pg (C), with a G + C content of 57.1%. The DNA content was less than that of Arabidopsis thaliana. The frequency of transformation by Agrobacterium tumefaciens using a binary vector plasmid pBI121 in the presence of acetosyringone was approximately 10%. GUS expression analysis and Southern blotting analysis of the genomic DNA of transformants revealed that all regions of T-DNA on plasmid pBI121 were integrated into the genome of M. polymorpha
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