A partial sequence of lipoxygenase gene from rough lemon (Citrus jambhiri L.)
1999
Sano, A. (Kagawa Univ., Miki (Japan). Faculty of Agriculture) | Akimitsu, K. | Yamamoto, H.
A full sequence of lipoxygenase (LOX) genes were available from various plants, however, nucleonic or enzymatic examinations had not been reported for any citrus genera nor species. In this study, we have amplified the partial region of transcripts encoding LOX of rough lemon (Citrus jambhiri L.) by reverse transcription-polymerase chain reaction (RT-PCR) with several primers designed from the alignment analysis of published plant LOX genes. The sequence of partial LOX coding region from rough lemon transcripts was identified by a direct sequencing from the RT-PCR product (601 bp), and compared for homology with other plant LOX genes. Blast analysis indicated that the RT-PCR products encoding rough lemon LOX gene were homologous to other plant LOX genes known, and higher homology was found toward the defense reaction related LOX genes of Arabidopsis or rice
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