Antioxidative lignans from industrial wastewater in cleaning of black sesame seed
1999
Nagashima, M. (Ichimura Gakuen Coll., Inuyama, Aichi (Japan)) | Fukuda, Y. | Ito, R.
The increase in industrial waste is one of the serious social problems. In this respect, we have searched for any useful materials from wastewater in cleaning of black sesame seed, one of food industrial wastes. In this paper, we describe the isolation, the structural elucidation and the antioxidative activity of four lignans, compounds 1-4, from the wastewater and on HPLC analysis of water extracts of black sesame seed coat and white sesame seed coat. Compounds 1-4 were isolated by column chromatography and preparative HPLC. On the basis of spectroscopic evidence, compounds 1-4 were respectively identified as pinoresinol, larisiresinol, hydroxymatairesinol, allohydroxymatairesinol. Compounds 2, 3, 4 have not been detected in sesame seed. On antioxidative activity by the thiocyanate method with AAPH, compounds 1-4 showed the weaker activities than BHT. On the DPPH radical-scavenging activities by a colorimetric method, compound 3 was as effective as alpha-tocopherol, and compound 4 showed the stronger activity than alpha-tocopherol. By HPLC analysis, it was ascertained that compounds 1-4 were not artifacts but were originally present in black sesame seed coat, in addition, it was proved that the content of compounds 1 and 2 in black sesame seed coat was four times more than that in white sesame seed coat
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