Characterization of the third alpha-amylase inhibitor, alphaAI-3, in the common bean (Phaseolus vulgaris L.)
1999
Suzuki, K. (Tsukuba Agricultural Extension Center, Ibaraki (Japan)) | Ishimoto, M.
Seeds of the common bean (Phaseolus vulgaris L.) contain proteinous alpha-amylase inhibitor (aAI) that inhibits insect and mammalian alpha-amylase enzymes. Based on the specific inhibition of alpha-amylase enzymes, common beans were classified into four different aAI types, aAI-1, 2, 3, and null type. This trait is controlled by a single locus, and alleles encoding the three aAI variants, aAI-1, 2, and -3, are codominant. Two different inhibitor proteins were purified from a common bean cultivar containing aAI-3. The two proteins are encoded at the same locus and designated aAI-3a and aAI-3b. aAI-3a inhibited the activity of porcine pancreatic alpha-amylase, while aAI-3b inhibited the activity of Mexican bean weevil larvae. N-terminal sequences and the specificity of aAI-3a and aAI-3b were very similar to aAI-1 and aAI-2, respectively, indicating that the common bean classified as aAI-3 type contains both analogs of aAI-1 and aAI-2
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