Responses of Mentha suspension-cultured cells to 2,4-dichlorophenoxyacetic acid and accumulation of esterified phenolic acids in their cell walls
1999
Yang, J.G. (Niigata Univ. (Japan)) | Miyao, S. | Uchiyama, T.
Two distinct types of cell growth of suspension-cultured Mentha were formed when the cells maintained in the medium containing 1000 mu-gI(-1) 2,4-D were subcultured into different 2,4-D concentrations. Few cell elongation of Mentha (average cell length: 34-40 mu-m) was observed after division in the medium containing 1-200 mu-gI(-1) 2,4-D; and significant cell elongation (average cell length: 95-130 mu-m) was observed after cell division in the medium containing 500-2000 mu-gI(-1) 2,4-D. A close correlation between culture medium and water content in the cells indicated that 2,4-D promoted cell elongation by water uptake. Amounts of phenolic acid in cell walls were much higher in unelongated cell walls than in elongated ones during the cultivation, and there was a close correlation between the amounts and the level of PAL activity in elongated and unelongated cells. However, there was no significant difference in cell wall components and its neutral sugar composition between elongat
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