Rice [Oryza sativa] mutant lines lacking alpha-globulin
1998
Iida, S. (Institute of Radiation Breeding, Omiya, Ibaraki (Japan)) | Miyahara, K. | Nishio, T.
In the screening of rice mutants having altered seed protein compositions, two mutant lines lacking alpha-globulin were selected from the M2 seeds of a white panicle mutant of cv. Koshihikari irradiated with gamma-rays. The mutant character, lack of alpha-globulin, was found to be controlled by a single recessive gene. Northern blot analysis of endosperm RNA using the probe of alpha-globulin cDNA revealed that the alpha-globulin gene is not expressed tin the alpha-globulin-less mutants. The mutated gene was considered to be alpha-globulin gene, and named glb-I(t). RFLP analysis of F2 plants between the mutant and indica cultivar "Kasalath" using land marker probes suggested that glb-I(t) is on chromosome 5 and that the distances of glb-I(t) from the markers, C246 and XNpb366, were 15.4cM and 30.8cM, respectively. The mutant line lacking alpha-globulin was expected to have a higher content of lysine in rice grains, but the lysine content of the mutant line was found not to be higher than the original cultivar. The possible use of the mutant as a breeding material is discussed
显示更多 [+] 显示较少 [-]