Establishment of a diagnostic method for porcine proliferative enteropathy using polymerase chain reaction
1999
Lym, S.K. | Lee, H.S. | Woo, S.R. | Yoon, S.S. | Moon, O.K. | Lee, Y.Y. (Ministry of Agriculture & Forestry, Anyang (Korea Republic). National Veterinry Research & Quarantine Service) | Koh, H.B. (National University Kwangju (Korea Republic). College of Betrinary Medicine)
Porcine Proliferative Ebterophthy(PPE) is an infectious enteric disease and a major cause of economic loss in swine industry due to weight loss, poor growth and sudden death in growing and finishing pigs at 6 tp 20 weeks of age. PPE has been diagnosed by clinical sighs, syndrom and lesions in the intestine in Korea. However, the diagnostic method had several problems in the detection of infected or carrier pigs. Therefore, in this study, we established the polymerase chain reaction(PCR) which ws a fast, specific and sensitive method for identification of Lawsonia intracellularis(L intracellularis). We designed and synthesized primer on the 16S rDNA and p78 gene encoding L intracellularis. Specificity of the method was confirmed by comparison of the PCR results using other enteric bacteria and the study has shown that PCR method was sensitive to detect 1ng of genomic DNA as a template. Identity of the PCR products was confirmed by comparison of pattern of restriction endonclease analysis with restriction enzyme HaeIII and PstI. Also, the PCR method was applicable to the naturally affected pigs with PPE. Based on the results from this study, the PCR method could be used as a fast and specific diagnostic tool for PPE.
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