Effects of glutamine, glycine and taurine on the development of in vitro fertilized bovine zygotes in a chemically defined medium
1998
Takahashi, Y. (Hokkaido Univ., Sapporo (Japan)) | Kanagawa, H.
The effects of glutamine, glycine and taurine on the development of bovine zygotes derived from IVM/IVF were determined using a protein-free chemically defined medium. After the cumulus cells were removed at 18 hr post-insemination, the presumptive zygotes were cultured for 4 or 6 days (about 104 or 154 hr) under gas atmosphere of 5% O2:5% CO2:90% N2. A modified synthetic oviduct fluid medium supplemented with 20 amino acids, insulin, and PVA was used as a basic medium (mSOFai). Omitting 1 mM glutamine from mSOFai did not affect the embryonic development after 4 and 6 days of culture. After 4 days of culture,no significant effects of glycine and taurine on the development of zygotes to the morula stage were observed. However, supplimentation with glycine or taurine significantly (P0.05) affected, with no interaction, the embryonic development to blastocysts after 6 days of culture. Addition of 5 mM glycine and 2 or 10 mM taurine significantly (P0.05) increased the percentage of blastocysts. The mean cell number in the blastocysts was affected by the glycine level, and was increased by the addition of 10 mM glycine (P0.001). These results demonstrate that glycine and taurine in a chemically defined medium containing a group of essential and non-essential amino acids improve the development of bovine zygotes to the blastocysts stage under 5% O2
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