Diagnosis of canine distemper by in situ hybridization
1999
Cho, H. | Park, N.Y. | Kim, Y.H. | Cho, K.O. | Park, H.S. | Park, Y.S. | Lee, B.J. | Chung, C.Y. | Im, H.H. (Chonnam National University, Kwangju (Korea Republic). College of Veterinary Medicine)
We have developed in situ hybridization(ISH) technique for rapid diagnosis of canine distemper(CD) which is the major infectious disease in dogs. In our experiment, we rapidly detected distribution of the specific canine distemper viral genome without disrupting morphology of tissues of cells. Two oligonucleotide probes for ISH were synthesized chemically and labelled 5' end with nonisotopic biotin by DNA synthesizer. The whole procedures of ISH was completed within 1~2 hours using the Microcapillary action system. On histological study, typical cytoplasmic or intranuclear inclusion bodies wer observed in the trachea, bronchiole, brain, and urinary bladder with the presence of prominent red positive signals on ISH, indicating specific CDV genome from the paraffin-embedded tissues of infected 13 cases. The results showed ISH can be used as a rapid and effective diagnostic method for diagnosis of CD.
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