Characterization of 30-kDa fragments derived from beta-conglycinin degradation process during germination and seedling growth of soybean [Glycine max]
1997
Kawai, M. (Ajinomoto Co. Inc., Kawasaki, Kanagawa (Japan). Central Research Labs.) | Suzuki, S. | Asano, M. | Miwa, T. | Shibai, H.
The degradation process of beta-conglycinin, a vicilin-type glycosylated storage protein of soybean seeds, during germination and seedling growth was examined by concanavalin A blotting combined with polyacrylamide gel electrophoresis. We detected and analyzed the structures of key intermediary fragments of 30 kDa derived from beta-conglycinin degradation, they were proved to be single-domain type subunits of beta-conglycinin. We show here a degradation process of beta-conglycinin: beta-conglycinin is subjected to limited proteolysis at exposed regions on the molecular surface, like domain junctions, generating 30-kDa single-domain fragments before non-specific proteolysis
显示更多 [+] 显示较少 [-]