Varietal variations in plant regenerative potential from protoplasts in spinach (Spinacia oleracea L.)
1998
Goto, T. (Toyo Inst. of Food Technology, Kawanishi, Hyogo (Japan)) | Miyazaki, M. | Oku, M.
The regenerative potential of seven Spinacia oleracea L. varieties in protoplast culture was compared. Although protoplasts were isolated from all cultivars, the yield of protoplast, plating efficiency (PE), and colony formation ratio wee remarkably different among them. The highest PE and colony formation ratio were obtained from protoplasts of "Ujo", followed by "Jiromaru", "Hoyo", and "Minsterland", which exceeded those of "Sapporo-oba", "Viroflay", and "Viking". Shoots were regenerated by "Ujo", "Jiromaru", "Hoyo", and "Minsterland", "Hoyo" and "Jiromaru" had the highest frequency; no shoot developed from the calli of "Sapporo-oba", "Viroflay", and "Viking". The highest frequency of shoot regeneration was obtained in a media containing 1.0 or 10.0 mg.liter(-1) IBA, but at 10.0 mg.liter(-1) IBA or IAA, the shootlets were often vitrified and fasciated
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