Efficient hybridization between Lilium x elegance and L. longiflorum through in vitro ovary slice culture
1998
Arzate-Fernandez, A.M. (Kyoto Univ. (Japan)) | Tanisaka, T. | Nakazaki, T. | Ikehashi, H.
Successful hybridization between Lilium x elegance and L. longiflorum has not been reported, although its reciprocal cross has been developed. Ovary slice culture technique, after cut-style pollination, was used to produce interspecific hybrids between these species. Crosses between diploid cultivars (2n=2x=24) of Lilium x elegance and L. longiflorum were conducted. On the days 5, 10, 15, 20, 25, 30, 35, 40, and 45th after pollination (DAP) ovaries were sliced and cultured on a modified MS (Murashige and Skoog 1962) medium without growth regulators and ammonium nitrate (HN4NO3), supplemented with 6% sucrose, 50 mg/l yeast extract and 0.25% gelrite at pH 6.3. Embryo germination was found only at 35 DAP. After transfer to a MS (half-strength) medium supplemented with 1.5% sucrose, 0.25% gelrite and 0.2% active charcoal at pH 5.8, seven hybrid plants were developed. All regenerated plants were identified as hybrids on the basis of karyotype, isozyme and PCR analyses
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