Restriction cleavage patterns of plasmid DNA of intermediately virulent Rhodococcus equi isolates from the mandibular lymph nodes of pigs in Kagoshima, Aomori and Miyagi perfectures [Japan] and the environment of pig-breeding farms
1999
Fukunaga, N. (Kagoshima-ken. Government Office (Japan)) | Okoda, T. | Katsumi, M. | Takai, S.
Rhodococcus equi (R. equi) was isolated from 4.6 and 4.1% of the mandibular lymph nodes of pigs in Kagoshima and Aomori Prefectures. Virulence of the isolates was investigated by means of immu-noblotting with a monoclonal antibody against a 20-kDa virulence-associated antigen. Most isolates were intermediately virulent manifesting a 20-kDa antigen. As a result of analysis by means of plasmid-DNA restriction cleavage patterns, isolates with the 20-kDa antigen from Kagoshima, Aomori and Miyagi Prefectures were divided into 8 types. Three of the 8 types were observed commonly in the isolates of 3 Prefectures. Of Kagoshima isolates, 94.9%, 63.1% of Aomori isolates and 74.7% of Miyagi isolates were typed as these 3 types. Intermediately virulent R. equi was isolated from the environment of a pig-breeding farm in Kagoshima Prefecture and one plasmid type of soil isolates was proved identical with that of isolates from pigs on the farm. This investigation shows that intermediately virulent R. equi was almost equally prevalent in the mandibular lymph nodes of pigs in Kagoshima, Aomori and Miyagi Prefectures. The role of soil might be significant in distribution of intermediately virulent R. equi in pig-breeding farms
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