Induced spawning of Paphia sp. using four chemicals
1998
Aguilar, C. | Amolo, R. | Sembrano, M. | Teng, A. | Ilano, A. (University of San Carlos, 6000 Cebu City (Philippines). Marine Biology Section)
The `short-necked clam', Paphia textilis, is one of the commercially viable food organisms that is feasible for studies and development. It is also referred to as "baby clams" or "carpet shell". In Bantayan Island, Cebu [Philippines], where this species was collected for this study, it is known as the `nailon clam'. Three chemicals (H2O2, NH4OH, KCl) were used as spawning induction agents. For each spawning agent, four concentrations were prepared with three replicates each. After some period in fresh seawater a 1 mL disposable syringe was used to inject the chemicals directly to the foot tissue of the clams. The following were recorded: latent period of spawning reactions and the number of gametes released. Counting of gametes was done using Sedgewick-Rafter counting chamber for ova, and a haemacytometer for the spermatozoa. Spawning agents (H2O2, KCL, NH4OH) used were effective in inducing release of gametes from the bivalve. Clams injected with KCl released a high density of sperm per mL, ranging from 2.6 x 10E5 to 3 x 10E5 sperms/mL in the four concentrations. Generally, it was found that higher cell density was directly proportional to the latent period of spawning reaction. Data showed that H2O2 attained a shorter latent period of spawning reaction
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