Determination of glutamate from fermentation process by carbondioxide sensor
1998
Ongart Wathanachaiyingyong
Aim of this work is to construct a glutamate sensor for determining glutamate concentration. The biosensor measured signals generated by CO2 from the reaction catalysed by glutamate decarboxylase. Escherishia coli cell and cell extracts were immobilised and employed in enzyme electrodes. The immobilisation methods used in this study were physical entrapment of enzyme/cell in clacium alginate and covalent binding of enzyme/cell by glutaraldehyde on permeable membranes. The electrode prepared from covalently bound enzyme was effective when measurement was done at 25 deg C in 100 mM pH 5.4 of citrate buffer containing 6.67*10*[-5) M pyridoxal phosphate. An electrode singnal is linearly related to the concentration of glutamate ranging from 125 to 1,000 milligrams per. liter. The peak of signals were reached within 2 minutes. Moreover, the electrode are high specific and stable. The life time is more than 28 days when stored at 4 deg C in the same conditions. Correlation coefficient between concentrations of glutamate obtained from enzyme electrode and from spectrophotometric method was 0.9969. The enzyme electrode can measure glutamate from fermentation broths of Corynebacterium glutamicum.
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