Establishment of embryogenic soybean suspension cultures
1990
Siranut Lamseejan | Arunee Wongpiyasatid | Sumin Smutkupt (Kasetsart Univ. Bangkok (Thailand). Faculty of Science. Dept. of Applied Radiation and Isotopes)
Immature cotyledons of soybean were placed on the medium containing MS (salts), B5 (vitamins) and 10 - 40 mg/l 2, 4-D. High percentage of embryogenesis was observed on the medium which contained 20 mg/l 2, 4-D. The medium which contained 3 percent sucrose gave higher percentage of embryogenic than the medium containing 6 percent sucrose. Embryos and embryogenic callus produced on the medium containing 20 and 30 mg/l 2, 4-D were placed into suspension culture medium containing MS (salts), B5 (vitamins), 15 mM glutamine, 5 and 10 mg/l 2, 4-D and 6 percent sucrose. Embryogenic suspension cultures proliferated as clumps of globular embryos and were maintained by subculturing every four weeks for over 6 months. Embryo development was observed when larger clumps of globular embryos were transferred to the MM medium for embryo maturation
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