Identification and determination of hypericine in the extracts of the amber - Hypericum perforatum L. ssp. angustifolium
2000
Smelcerovic, A.A. (Faculty of Technology, Leskovac (Yugoslavia)) | Djordjevic, S.M. | Gudzic, B.T. | Lepojevic, Z.D.
The extraction of plant species Hypericum perforatum L. ssp. angustifolium (amber) was carried out by the following methods: maceration, ultrasonic maceration and extraction with CO2 under high pressure. The highest yields of dry extract were achieved by the ultrasonic maceration method (23.3%) and the conventional maceration method (22.9%). Freytag's HPLC method was used to determine the hypericine content in methanol ultrasonic macerate (0.039%) and in methanol macerate (0.036%). In the extracts obtained by extraction by CO2 under high pressure no traces of hypericine were found. The results obtained indicate that the most convenient method for the extraction of hypericine is ultrasonic maceration with methanol as the extraction agent.
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