Polymorphism of protein markers in Serbian spruce [Picea omorika] half-sib lines
2000
Sijacic-Nikolic, M. (Univerzitet u Beogradu, Beograd (Yugoslavia). Sumarski fakultet) | Isajev, V. | Konstantinov, K. | Mladenovic-Drinic, S.
This paper present the results of determination of the degree of genetic polymorphism of Serbian spruce (Picea omorika) half-sib lines belonging to different varieties: var. semidihotomy, var. serbica and var. nana, incorporated in the seedling seed orchards, by the application of protein markers. The analysis in the salts of soluble proteins of the endosperms of 12 Serbian spruce (Picea omorika) genotypes was performed by separation - electrophoresis on the polyacrylamide gel using Leammli (1970) method. The obtained electrophoregrams were the base for the calculation of the coefficient of similarity, by Sheen (1972) formula, both between individuals within the lines and between the lines. THe genetic distance of the analysed material was assessed by cluster analysis. The study results show that phenotypic variability between the analysed varieties of Serbian spruce (Picea omorika) is genetically determined.
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