Use of molecular markers for the identification of apple varieties and rootstocks (Malus domestica [L.] Borkh.) | Utilisation de marqueurs moleculaires pour l'identification de varietes et de sujets porte-greffe de pommier (Malus domestica [L.] Borkh.)
2001
Tignon, M.
Efficient molecular tools were developed for the identification and certification of about fifty apple varieties and rootstocks from the Belgian cultivar panel. The RFLP method allowed the distinction of 45 from the 49 tested apple varieties, on basis of 21 polymorphic fragments revealed by three probe-restriction enzyme combinations. The AFLP technique allowed the distinction of all the tested varieties, on basis of 46 polymorphic fragments revealed by two amplification profiles. Its efficiency is similar to that of the "Simple Sequence Repeats" technique in the distinction of apple cultivars. However, the AFLP technique didn't permit the distinction of color mutants from the Jonagold variety. Nevertheless, it revealed an existing genetic instability of this variety and its tested mutants. Besides, the ® differential display¯ technique, applied to messenger RNA extracted from fruit epidermis, didn't reveal expression polymorphism that can be used for the distinction of the studied mutants. However, this technique highlighted the presence of an apple gene similar to that coding for the reverse transcriptase from a copia retrotransposon. It was briefly identify and characterized in apple as well as different plant species representing the Rosaceae family
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