Genetic recombination in lactobacilli
2001
Denkova, Z. | Mirgov, I. (Higher Institute of Food and Flavour Industies, Plovdiv (Bulgaria))
An investigation genetic recombination by fusion of protoplasts of antibiotic-resistant clones of lactobacilli: Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus helveticus, Lactobacillus casei, and Lctobacillus plantarum has been accomplished. It has been shown that the conditions of degradation of the lactobacilli cell wall allow a high relative share of formed protoplasts to be achieved. Aftre the protoplast fusion and regeneration of the cell wall a heterogenic population is obtained. A series of recombinant clones has been selected, which differ from the parent cultures in improved or new qualities, such as higher reproducibility, acid-forming ability, asimilation and fermentation of a greater number of carbon sources, phage-resistance etc. Among them have been selected the recombinant strains - Lactobacillus LAB 8, Lactobacillus LHC 28, Lactobacillus LBC 7, Lactobacillus LBC 16, Lactobacillus LPC 12, Lactobacillus LPC 48, Lactobacillus LPC 51.
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