Analysis of some endogenous plant hormones during induction of somatic embryogenesis in common bean (Phaseolus vulgaris L.)
2001
Dobrev, P. | Petkov, P. (AgroBioInstitute, Kostinbrod (Bulgaria)) | Svetleva, D. | Ivanova, A. | Djilianov, D | Petkova, S. | Atanassov, A.
The contents of IAA and ACC in different explants like globular structures, residual explants and cultural media were investigated, during induction of somatic embriogenesis in common bean: variety Dobrudjanski 7 and the mutant one, Plovdiv 11M. Somatic embriogenesis was induced in liquid media B5, containing 2,4-D in concentrations of 1 and 2 mg/l. The endogenous content of IAA varied in dependence of the genotype, explant's type and their age. The concentration of exogenous applied auxin 2,4-D and the endogenous content of IAA in the induced globular structures were inversely related. In spite of the exogenous auxin concentrations the embryos of Dobrudjanski 7 variety contained more IAA. The concentration of 2,4-D and the content of IAA in the media, at the moment of somatic embriogenesis induction were positively related.
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