The use of cytochrome P450 monooxygenase genes to introduce herbicide tolerance in tobacco
2001
Yordanova, E. | Gorinova, N. | Atanassov, A. (AgroBioInstitute, Kostinbrod (Bulgaria))
Cytochrome P450 monooxygenases play an important role in detoxification and/or activation of a large number of xenobiotics including pesticides. The recovery of an increasing number of cytochrome P450 genes has enabled their use in experimentation that has revealed their extraordinary potential for engineering herbicide tolerance, biosafening, bioremediation and green chemistry. The transgenic nature of both transgenic plants was proved by kanamycin resistance, PCR analysis and herbicide tolerance. Chlortoluron-resistant tobacco plants are self-pollinated and the segregation of resistance to herbicide was followed up to R2 generation. The herbicide chlortoluron at doses 0.1-0.5 mM decreased the percent of generation of O. ramosa seeds anf the full inhibition was observed at dose 12.5 mM. The obtained transgenic tabacco plants carrying the fused rat cytochrome P4501A1/yearst reductase gene from R2 progeny were used to investigate the effeciency of chlortoluron for broomrape control in pots. According to our results the broomrape on transgenic tobacco plants treated with 0.5 mM chlortoluron developed 3-4 weeks later than did the nontreated control and transgenic tobacco plants.
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