Effect of plant oils and malate on rumen fermentation in vitro
2002
Jalc, D. (Slovenska Akademia Vied, Kosice (Slovak Republic). Ustav Fyziologie Hospodarskych Zvierat) | Ceresnakova, Z.
The objective of this study was to determine the effect of rapeseed (RO), sunflower (SO) and linseed (LO) oils (10 % wt/wt) and maleic acid, disodium salt (MAL; 8 mM) on the rumen fermentation of diet (250 mg) consisting of hay, barley and sugarbeet molasses (60:30:10). Rumen fluid was collected from two sheep (Slovak Merino) fed with the same diet twice a day. Mixed ruminal microorganisms were incubated in fermentation fluid (40 mL) which contained 20 % vol/vol rumen fluid and McDougall's buffer. Incubations were carried out in batch cultures at 39 deg C for 72 h two times within 3-weeks intervals. Compared to control, all supplemented diets (RO, SO, LO, MAL) significantly increased pH (P 0.001), the concentrations of propionate, SO, RO, MAL, acetate to propionate ratio and decreased methane production. The additives - plant oils, not malate, significantly decreased lactate and in vitro dry matter digestibility. Incorporation of plant oils into malate-treated incubated feeds negated the decrease of n-butyrate, lactate and the increase of pH and ammonia associated with malate treatment. The effect of combined additives (RO+MAL, SO+MAL, LO+MAL) on methane and propionate productions was less effective. All combined additives caused significant decrease of feed digestibility. Combination of the additives did not show an additive effect on rumen fermentation.
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