Differences in the microflora of scarified and unscarified seeds of Karwinskia humboldtiana (Rhamnaceae)
2001
Henselova, M. (Univerzita Komenskeho, Bratislava (Slovak Republic). Katedra Fyziologie Rastlin) | Hudecova, D.
Seeds of Karwinskia humboldtiana obtained from a 1997 collection in the locality of Villa de Garcia Nuevo (Leon, Mexico) were contaminated with spores of filamentous fungi, bacteria and yeasts. The concentration of microorganisms in unscarified seeds ranged from 3.0 x 10**3 to 7.5 x 10**3 CFU/g. Bacterial isolates of the genera Aeromonas sp., Bacillus, and Pseudomonas were predominant. The following filamentous fungi were identified: Alternaria, Aspergillus niger, Cladosporium sp., Fusarium sp., Mucor sp., Penicillium commune, Trichothecium sp.; from yeasts Rhodotorula sp. and Saccharomyces cerevisiae. Seed scarification significantly reduced the microbial contamination. Only two fungi, Cladosporium sp. and Saccharomyces cerevisiae, out of the original fungi isolates were identified on scarified seeds. Although a relatively high incidence of an unidentifiable species of Penicillium sp. was found, the bacterial spectrum was not altered. Treatment of scarified seeds with Vitavax 200 WP and Pomarsol Forte 80 WP (3 mg/g seeds) augmented germination by 10-19 % compared to treated unscarified seeds, and by 16-31 % compared to untreated unscarified seeds.
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