Analysis of relations between polymorphism in steroid 21-hydroxylase gene (CYP21) and quantitative and qualitative characters of boar semen
2002
Kmiec, M. | Ziemak, J. | Dybus, A. | Matusiak, S. (Agricultural Univ., Szczecin (Poland))
The purpose of this study was to find a relation between the frequency of mutation in the CYP21 gene in boars and qualitative and quantitative characters of the semen under study. The study included 88 boars from which the semen was taken to inseminate sows. The CYP21 polymorphism was determined by means of the PCR-RFLP method. The amplification product was digested with the HaeIII endonuclease. Two alleles of the CYP21 were identified: CYP21**A allele - 448 bp and CYP21**B allele - 350 and 98 bp. The frequency of CYP21**A was 0.1989, whereas that of CYP21**B was 0.8011. Two CYP21 genotypes were found in the boars under study. The frequency of the AB genotype was 0.3978 and that of BB was 0.6022. An analysis of the relation between the CYP21 genotypes and boar semen characters showed statistically significant predominance of boars with the AB genotype in terms of the percentage of normozoosperms, number of normozoosperms in a dose and higher number of sperm cells in the insemination dose.
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