Accumulation of microsomal polypeptides in barley roots during aluminium stress
2002
Mistrik, I. | Tamas, L. | Huttova, J. | Gejdosova, V. (Slovenska Akademia Ved, Bratislava (Slovak Republic). Ustav Botaniky)
Accumulation of two peripheral membrane polypeptides (20 and 28 kDa) in roots of Al-sensitive (cv. Alfor) and Al-resistant (cv. Bavaria) barley cultivars were analysed during Al stress. Both cultivars were subjected to Al concen-tration ranging from 0 to 150 microM for 24, 48, 72 and 96 hours. Accumulation of both polypeptides was determined 24 h after duration of Al treatment. Although, based on root growth test, Bavaria showed significantly greater resistance to Al than Alfor, analysis of 20 and 28 kDa polypeptide pattern has not revealed significant difference between the two cultivars. However, accumulation of 20 and 28 kDa polypeptides in Alfor was selectively induced by Al treatment because different pH of the root media (pH 3.5 to 6.5) or application of other metals (Cu, Co, or Cd) failed to induce these two bands. On the other hand, accumulation of these polypeptides in Bavaria was induced not only by Al, but also by Cd and in a lesser extent by Co treatment.
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