In vitro transfer of L-alanine, L-histidine and carnosine across the rumen epithelium of sheep
2001
Faix, S. (Slovenska Akademia Vied, Kosice (Slovak Republic). Ustav Fyziologie Hospodarskych Zvierat) | Faixova, Z.
The objectives of this experiment were to study the transfer of free amino acids (alanine, histidine) and dipeptide (carnosine) of different concentrations from the mucosal to the serosal side of the rumen epithelium after 60 min of incubation in vitro. Experiments were carried out on the rumen epithelium taken from twenty four young sheep. The transfer of alanine and carnosine was not significantly different between concentrations of 0.5 and 1 mmol/L. Only the transfer of histidine increased in both cases from 0.036 +/- 0.007 to 0.074 +/- 0.002 mmol/L. The transfer of histidine rose with increasing concentration from 1 to 5 or 10 mmol/L. Alanine increased from 1 to 5 mmol/L but the l0 mmol/L concentration did not affect the transfer of alanine. The opposite result was found in carnosine. The 5 mmol/L concentration did not affect the transfer of carnosine compared with 1 mmol/L but the transfer of carnosine was six times higher in the 10 mmol/L versus 5 mmol/L concentration. The results indicate that the transfer of amino acids through the rumen wall in lower initial concentrations is easier than the transfer of the dipeptide.
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