Isolation and characterization of a polygalacturonase-inhibiting protein from potato leaves. Accumulation in response to salicylic acid, wounding and infection | [Isolement et caracterisation d'un proteine des feuilles de pomme de terre qui inhibe la polygalacturonase. Accumulation en reponse a l'acide salicylique, aux blessures et aux infections]
2001
Machinandiarena, M.F. (Universidad Nacional de Mar del Plata (Argentine). Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Biologicas) | Olivieri, F.P. | Daleo, G.R. | Oliva, C.R.
A polygalacturonase-inhibiting protein (PGIP) was purified from potato leaves (Solanum tuberosum L. cv. Spunta). The protein was cell wall bound and had a molecular mass of 41 kDa, as determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and gel filtration. Potato PGIP showed a broad inhibitory activity against crude polygalacturonase preparations from several fungi grown in liquid culture. Using heterologous antibodies in western blot experiments, it was shown that the levels of PGIP are increased in potato leaves in response to wounding, treatment with salicylic acid and infection with Phytophthora infestans during an incompatible interaction. The data support the hypothesis that synthesis of PGIP may constitute an active defence mechanism of potato plants that is elicited by signal molecules known to induce plant defence genes
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