Isolation and Determination of Phenolic compounds in peony (Paeonia lactiflora Pall.) Root
2000
Kang Kwang-Hee | An Young-Nam Yeungnam Univ., Kyongsan (Korea Republic)
The five phenolic compounds of peony root were isolated by Sephadex LH-20 column chromatography. their chemical structures were identified by spectroscopic methods (UV, FT-IR, FAB-MS and 1H.13C-NMR). The complete structures of these compounds were elucidated to be (+)-taxifolin-3-O-beta-D-glucopyranoside, benzoic acid, gallic acid, (-)-epicatechin and (+)-catechin. The concentrations of five phenolic compounds in the peony root of three Korean cultivars (Youngchonjakyak, Euisungjakyak and Jomjakyak) were determined by reverse-phase HPLC. The constituents concentration in Youngchonjakyak were generally higher than in Euisungjakyak and Jomjakyak. The concentrations of (+)-taxifolin-3-O-beta-D-glucopyranoside, benzoic acid, gallic acid, (-)-epicatechin and (+)-catechin in three different cultivars were ranged 0.23~0.52%, 0.20~0.30%, 0.26~0.28%, 0.09~0.12% and 0.34~0.63%, respectively.
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