Isolation of Alkalophilic bacillus sp. KJ-133 Producing Cyclomaltodextrinase and Its Enzyme Production.
2000
Jung H.J. | Kwon H.J.(King sejong University, Seoul, Korea Republic)
To produce and utilize micorbial cyclomaltodextrinase benig industrially useful, we isolated an alkaophilic Bacillus strain from soil which was capableof degrading cyclodextrins. The newly isolated strain was aerobic, gram-positive, spore-forming, motile, rod shape(0.2~0.4*1.4~4.4 mum),and 35.8 mol% of DNA base composition. Based on its morphological, physiological, and biochemical properties, it was identified as alkalophilic Bacillus sp. KJ-133 and cultivated well in the ranges of 30~40 degrees and pH 8.0~9.0. The cyclomaltodextrinase og the strain showed maximal production after 48 h of cultivation at 37 degrees, and the activity was inhibited by Ag2+, Hg2+, Cu2+, and p-cholromercuribenzoate.
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