Recovery of Paclitaxel from Suspension Culture Medium with Hydrophobic Resin
2000
Kim Jin-Hyun | Hong Seung-Suh Samyang Genex Biotech Research Institute, Taejon (Korea Republic)
The soluble paclitaxel was found in the supernatant of the plant cell cultures of Taxus chinensis. The percentage of soluble paclitaxel depends on paclitaxel concentration in bioreactor. As paclitaxel concentration decreases, the percentage of soluble paclitaxel increases. It is therefore important ot develop a new process for the recovery of soluble paclitaxel. The use of hydrophobic resin, HP20, gives nearly perfect recovery of paclitaxel in supernatant. The resin was more effective in treatment of the cell and debris free filtrate, probably because of the reduced solids content. In this case, 3 g/l resin and 1 day reaction were enough for recovery the soluble paclitaxel in medium.
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