Transgenic Tobacco Plants Expressing the Coat Protein of Cucumber Mosaic Virus Show Different Virus Resistance

Ryu Ki-Hyun; Park Won-Mok Graduate School of Biotechnology, Korea University, Seoul 136-701

Transgenic Tobacco Plants Expressing the Coat Protein of Cucumber Mosaic Virus Show Different Virus Resistance

1998

Ryu Ki-Hyun | Park Won-Mok Graduate School of Biotechnology, Korea University, Seoul 136-701 (Korea Republic)

Transgenic tobacco (Nicotiana tabacum cv. Xanthi-nc) plants were regenerated after cocultivation of leaf explants with Agrobacterium tumefaciens strain LBA4404 harboring a plasmid that contained the coat protein (CP) gene of cucumber mosaic virus (CMV-As). PCR and Southern blot analyses revealed that the CMV CP gene was successfully introduced into the genomic DNA of the transgenic tobacco plants. Transgenic plants (CP ion) expressing CP were obtained and used for screening the virus resistance. They could be categorized into three types after inoculation with the virus: virus-resistant, delay of symptom development, and susceptible type. Most of the CP ion transgenic tobacco plants failed to develop symptoms or showed systemic symptom development delayed for 5 to 42 days as compared to those of nontransgenic control plants after challenged with the same virus. However, some CP ion transgenic plants were highly susceptible after inoculation with the virus. Our results suggest that the CP-mediated viral resistance is readily applicable to CMV disease in other crops.

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