Effect of environmental factors on the growth and sporulation of Phytopthora nut rot in culture
2001
Bachiller, N.S.J. (Philippine Coconut Authority - Davao Research Center, Bago Oshiro, Davao City (Philippines))
Mycelial growth and sporulation of Phytopthora nut rot isolates were variously affected by different light exposure, pH levels, temperature and relative humidity. Culture grown under continuous light had the widest colony diameter (79.9 mm) and highest number of sporangia at 45.5 x 10 sq sporangia per ml, 7 days after inoculation while the smallest colony (41.4 mm) and lowest sporangia count (23 x 10 sq per ml) was produced under continuous darkness. Phytopthora nut rot isolates produced bigger colony diameter and produced more sporangia from pH 5.0 to 8.0. Mycelial growth and sporangial count were higher when the isolates were incubated at 25 deg C as compared to 30 deg C. Different levels of relative humidity (RH) significantly affected the percentage germination of Phytopthora nut rot isolates. Germination was positively correlated with the increase in RH. Highest germination (26.8%) was observed in 100% RH while lesser germination at 90% RH (1.5%) at 48 hours of incubation. After 72 hours of incubation at 100% and 97.5% RH germination of spores was 48.5 and 42.2% respectively
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