Optimization of seed germination and assessment of genetic diversity in Dalbergia sissoo Roxb. (Shisham)
2001
Dubey, P. | Shringirishi, N. | Shukla, N.K. | Saxena, S. | Srivastava, R.J. | Burfal, E.S. (Forest Research Inst., U.P. 18 - G.T. Road, Kanpur, (India))
Dalbergia sissoo Roxb. (Shisham) belongs to sub-family Papilionoidae of the Fabaceae family. It is an important tree for timber, fodder and fuelwood. This study was undertaken to determine seed source variation of this species and to standardize the germination techniques. Seeds were collected from thirteen seed sources. There was significant genetic variation among seed sources for seed morphological traits (seed length, seed width, seed thickness, 100 g seed weight), moisture content and germination percent. Differences in trait means and variances were found to be statistically significant among the populations. In the correlation studies of traits and geographical factors, germination percent displayed a significant positive correlation with latitude. Correlation studies among seed traits revealed that at the phenotypic level seed width showed a positive correlation with seed length. Moisture content had a negative association with seed thickness. At the genotypic level, the study indicated a positive significant association between seed width and seed length. The 100 g seed weight also gave significant positive correlation between seed length and width, while moisture content was significantly negative in its association with seed thickness and 100 g seed weight. The heritability in the broad sense was highest for germination percent (94.4) followed by seed thickness. The little difference between genotypic and phenotypic coefficient of variation indicates a neglible influence of the environment in the expression of these characters. Maximum germination for most of the seed sources occured between the germination paper and at temperature 30 deg C. Similarly, the maximum germination value was also obtained with the temperature and substrate. The Solan seed source had the highest germination percent and germination value
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