Microbiological analysis of an infant feed formula and a powdered full cream cow's milk
2000
Gutierrez, R.M. (Philippines Univ. Baguio, Gov. Pack Rd. Baguio City (Philippines) Dumol, C.G.
A microbiological analysis of an infant feed formula and a powdered full creaM cow's milk was undertaken to determine their bacterial load during storage and to relate it to their product quality and safety. Milk samples were prepared in infant feeding bottles according to instructions indicated in their labels. Using the standard plate count on nutrient agar, the number of bacterial colonies in milk samples were estimated for the different sampling periods (0 hour, and 1 hour intervals for the first 6 hours, then at 12, 24 and 48 hours of standing at room temperature). The methylene blue reduction test and pH measurements were also performed every sampling period. It was revealed in this study that the bacterial content of full cream cow's milk is initially higher than that of the infant feed formula. Bacterial count was 10,000 cfu/ml, but starting on the fourth hour, it significantly increased up to more than 20,000 cfu/ml. Full cream cow's milk is therefore safe to drink up to the third hour of standing at room temperature. Infant feed formula on the other hand, is safe to drink up to the sixth hour of standing at room temperature since its bacterial content is 10,000 cfu/ml. Scouring of milk samples started at the 24th hour of standing as indicated by the methylene blue reduction test and pH measurement. Majority of the colonies that grew in both types of milk were gram negative cocci or rods. It is imperative that the bacterial content of milk preparation often given by mothers to infants and older children be assessed, since it is not only important for the prevention of spoilage but also for the prevention of transmission of diseases
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