Development of rapid methods of detecting pesticide residues in stored grains
2001
Calumpang, S.M.F. (Philippines Univ. Los Banos, College, Laguna (Philippines). National Crop Protection Center) Morillo, G.G.L. | Flavier, M.E. | Santos, J.H.
In a continuing program to expand the applicability of rapid test kits (RTKs) produced by the National Crop Protection Center, methods were developed for the fast, semi-quantitative detection of residues of 4 insecticides belonging to 3 pesticide groups, i.e., deltamethrin and cypermethrin (both pyrethroids), malathion (an organophosphate), and carbaryl (a carbamate), used in stored farm produce, i.e., mungbean (Vigna radiata), corn (Zea mays), and rice (Oryza sativa) whether whole or milled. RTKs are necessary for quick analysis of commercial farm produce which may contain pesticide residues beyond the maximum residue limits (MRLs) standards set by the FAO-WHO-supported Codex Alimentarius Commission to protect consumers all over the world. With the new methods, residues could be detected at 2 mg/kg for deltamethrin, at 0.30 mg/kg for cypermethrin, and at 8 mg/kg for malathion in mungbean, corn, and rice grains. Carbaryl in mungbean could be detected at 5 mg/kg. The new rapid tests for malathion and carbaryl could easily detect residues equal to or higher than their Codex maximum residue limits (CXLs) in the grains, while those developed for deltamethrin and cypermethrin could detect residues at levels slightly higher than their respective CXLs. Residue levels detected using the rapid tests agreed closely with the theoretical spiking levels. Precision and accuracy of the detection methods were validated through coventional methods such as gas liquid chromatography or ultra violet spectrophotometry when appropriate
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