Fuminosin-producing ability of Fusarium spp. in Philippine corn
1996
Veluz, G.A. | Sanchez, P.C. | Dizon, E.T. (Philippines Univ. Los Banos, College, Laguna (Philippines). Inst. of Food Science and Technology) Yoshizawa, T.
Fusarium spp. were isolated from various corn samples collected in different parts of the Philippines following the single spore isolation technique. Sixty three corn culture materials (CM) were prepared by inoculating the isolated Fusarium spp. in corn. Twenty-six samples were incubated at 30 deg C for 14 days while 19 and 18 corn samples were incubated at 20 deg C and 22 deg C for 21 days, respectively. Extracted samples were analysed for the presence of fuminosins by Thin Layer Chromatography (TLC) and by High Performance Liquid Chromatographic (HPLC) method using OPA-derivatized samples and flourescence detection. Results showed that most of the sample extracts were positive for fuminosins B1 (FB1) and fuminosins B2 (FB2). HPLC revealed that high FB1 concentration ranging from 1072 ppm to 5390 ppm were observed in 14 CM incubated at 20 deg C. CM incubated at 30 deg C have relatively lower FB1 of less than 1000 ppm except for one isolate (B14) that produced 4700 ppm. No FB2 production was observed in many of the samples. CM subjected to 22 deg C had 143-1900 ppm FB1, and 0-292 ppm FB2. FB1:FB2 ratios varied differently from one condition to another ranging from 1.1 to 30.0, 2.6 to 17.1 and 5.6 to 15.9 for CM incubated at 30 deg C, 20 deg C and 22 deg C, respectively
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