Naturally occurring allelic variations as a new resource for functional genomics in rice.
2001
Yano, M.
Recent progress in rice genome analysis has made it possible to analyze naturally occurring allelic variation underlying complex traits. Using heading date as a model for complex traits, we detected and characterized quantitative trait loci (OTLs) and identified genes at QTLs at the molecular level. QTLs for heading date were mapped by using several types of progeny derived from a cross between varieties Nipponbare and Kasalath. Nine QTLs were mapped precisely as single Mendelian factors by the use of advanced backcross progeny. Nearly isogenic lines of QTLs were also developed by marker-assisted selection and were used to identify the function of each detected QTL. Combining two QTLs into the genetic background of Nipponbare allowed us to investigate epistatic gene interactions among QTLs. We analyzed a large segregating population by genetic and physical mapping to narrow down candidate genomic regions for target QTLs. These analyses revealed 10- to 50-kb regions as candidate regions for the target ge es. We indentified genes of the most probable candidates for the photoperiod-sensitivity loci Hd1 and Hd6. Naturally occurring allelic variation could be a new resource for the functional analysis of rice genes.
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