Isolation of bacteria highly sensitive to various antibiotics for using in detection of antibiotic residue in milk
1997
Suphachai Nuanualsuwan | Kriengsak Saitanu | Thongchai Chalermchaikit (Chulalongkorn Univ., Bangkok (Thailand). Faculty of Veterinary Science. Dept. of Veterinary Public Health)
Bacteria from 16 hot springs were isolated and used for detecting antibiotic residues. It was found that 2 isolates out of 145 cultures grown at 37 deg C and 4 isolates out of 61 cultures grown at 64 deg C could detect various kinds of antibiotic residues closed to the Maximum Residue Limit (MRL) set by WHO or almost sensitive as the standard strain, Bacillus stearothermophilus var, calidolactis C593. Using disk assay method, strain "4.2/64" isolated from Chiangrai province can detect ampicillin, streptomycin, kanamycin, gentamycin, erythromycin chloramphenicol and sulfamethoxazole at 0.02, 3.2, 4.8, 0.048, 0.32, 25.0, 3.125 micro g/ml, respectively.
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