Effects of a portacaval shunt on the enteroinsular axis in rats
2001
Radosavljevic, T. (Faculty of Veterinary Medicine, Belgrade (Yugoslavia). Institute of Pathophysiology) | Nikolic, I. | Todorovic, V. | Sikic, B. | Nikolic, J.A.
We investigated the effect of a portacaval shunt on the enteroinsular axis in rats, 8 weeks after surgery. Fasting plasma levels of insulin, glucagon, somatostatin and glucose, as well as pancreatic content of insulin, glucagon, and somatostatin were determined. In addition, determination of the fasting plasma level and concentration of GLP-1 in tissue extracts of ileum, as well as morphometric analysis of GLP-1-immunoreactive cells in different segments of the small intestine were included. Normal plasma levels of insulin and somatostatin, accompanied by hyperglucagonemia, and hypoglucemia were observed. Plasma level of GLP-1, and the number of GLP-1-immunoreactive cells in ileum were significantly reduced in rats with a portacaval shunt compared with control animals. However, concentrations of GLP-1 in tissue extracts of the ileum of portacaval shunted rats were unchanged. These results suggest that the abnormalities of the enteroinsular axis present in rats with a portacaval shunt were due to the functional deterioration of the liver.
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