Streptomyces subtilisine like (SSL) inhibitor from Streptomyces hygroscopicus CH-7: detection and partial characterization
2000
Izrael, L. (University of Belgrade, Belgrade (Yugoslavia). School of Medicine, Department of Chemistry) | Karadzic, I. | Gojgic-Cvijovic, G. | Vrvic, M.M.
Strain Streptomyces (S.) hygroscopicus CH-7 during the fermentation of polyketide antibiotic produces as a by product proteolytic complex with significant trypsin like activity. Also, S. hygroscopicus CH-7 produces extracellulary proteinaceous protease inhibitor known as Streptomyces subtilisine like inhibitor (SSL-inhibitor). Detection of SSL-inhibitor is performed on the base of stability of SSL-inhibitors on very low pH (final concentration of TCA is 9%). Molecular mass of SSL-inhibitor is approximately 11 kDa, and it provides very wide range of inhibitory activity toward different serin proteases: trypsin, protease from Bacillus (B.) subtilis, proteinase K from Tritirachium (T.) album, protease from B. licheniformis, protease from S. griseus and the complex of proteases from S. hygroscopicus CH-7.
显示更多 [+] 显示较少 [-]