Effectiveness of six highly polymorphic microsatellite markers in resolving paternity cases in Thoroughbred horses in Slovakia
2002
Jakabova, D. | Trandzik, J. | Chrastina, J. | Hudecova, L. | Zetochova, E. | Kozlik, P. (Statny Plemenarsky Ustav, Nitra-Luzianky (Slovak Republic)) | Bulla, J. | Bugarsky, A. | Jakab, F.
Six microsatellite loci were evaluated for their suitability for parentage control in horses. A combination of PCR and semiautomatic fluorescence-based detection was applied. A total of 352 animals were tested. The microsatellite loci showed extensive polymorphism with allele numbers ranging from 7 to 11 and polymorphism information content (PIC) values in the range 0.47-0.82. The analysis of these loci also revealed that they had a 98.88 % combined probability of exclusion (PE) of false parentage. The results of this study suggested that a very high probability of exclusion could be reached with only six microsatellite loci.
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