Molecular diagnostics of porcine stress syndrome and occurrence of porcine stress syndrome in Estonia pig breeding enterprises
2003
Sabre, D. | Viinalass, H. | Vaerv, S. (Estonian Agricultural University, Tartu (Estonia))
A total of 477 pigs from eight Estonian pig breeding enterprises were genotyped for porcine stress syndrome and the occurrence of porcine stress syndrome was investigated. The DNA extraction method from hair roots by using 200 mM NaOH was developed. According to the results of PCR-RFLP analysis 91.0 per cent of pigs were stress negative (genotype NN), 8,6 per cent heterozygous carries (genotype Nn) and 0.4 per cent stress-positive (genotype nn). All genotyped pigs were stress-negative in three out of eight investigated pig breeding enterprises. The occurrence of heterozygous pigs for PSS was up to 22.9 per cent. The stress-positive pigs were found only in one pig breeding enterprise. The frequency of porcine stress syndrome varied from 0 to 0.115, on average 0.047. The relative low occurrence of PSS can be explained by selection of boars for implementing breeding programmes
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